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Wham clusters every one of the applicant breakpoints and rounds their positions to the closest tenth foundation pair. The position with the highest study assistance is noted. Should the comfortable-clipped consensus sequence can be aligned to your putative breakpoint area utilizing the Smith-Waterman algorithm, the breakpoint is further refined to
Wham is made for paired-end Illumina libraries with standard insert dimensions (~300bp-500bp). Wham integrates mate-pair mapping, break up study mapping, delicate-clipping, substitute alignment and consensus sequence primarily based evidence to predict SV breakpoints with solitary-nucleotide accuracy. Wham generates a merged pileup (catalog of read